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Updated: Aug 17, 2026
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Published on: July 4, 2007
The identification, cloning and functional expression of the gene encoding orotidine 5'-monophosphate (OMP)
R I Menz1, O Cinquin, R I Christopherson
1School of Biological Sciences, The Flinders University of South Australia, Bedford Park, SA 5042, Australia.
Abstract:
The coding region of a putative orotidine 5'-monophosphate decarboxylase gene from Plasmodium falciparum was identified in genomic data from the Malarial Genome Sequencing Project. The gene encodes a protein of 323 amino acids with a predicted molecular weight of 37.8 kDa. The gene was cloned into a bacterial expression vector and over-expressed in Escherichia coli. The recombinant protein was purified and shown to have orotidine 5'-monophosphate decarboxylase activity, confirming the identity of the gene.
