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Related Experiment Video

Updated: Jul 11, 2026

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Published on: October 25, 2011

HER-2/neu expression in germ cell tumours.

S Soule¹, L Baldridge, K Kirkpatrick

¹Division of Hematology and Oncology, Indiana University School of Medicine, Indianapolis 46202, USA. shsoule@iupui.edu

Journal of Clinical Pathology

|August 27, 2002

Summary

HER-2/neu protein overexpression in germ cell tumors is not reliably detected by immunohistochemistry (IHC) compared to fluorescence in situ hybridization (FISH). This lack of correlation suggests limited prognostic or therapeutic value for HER-2/neu in these cancers.

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Area of Science:

Oncology
Molecular Pathology
Cancer Genetics

Background:

HER-2/neu protein overexpression is a key target in some cancers.
The role of HER-2/neu in germ cell tumors (GCTs) requires further investigation.
Accurate detection methods are crucial for assessing therapeutic relevance.

Purpose of the Study:

To evaluate HER-2/neu positivity rates in GCTs using both immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH).
To determine the concordance between IHC and FISH results for HER-2/neu assessment in GCTs.
To ascertain the potential prognostic and therapeutic implications of HER-2/neu in GCTs.

Main Methods:

Analysis of 96 archival GCT pathology specimens.
HER-2/neu protein expression assessed by IHC using the HercepTest kit.
HER-2/neu gene amplification evaluated by FISH with the INFORM assay and a centromere 17 probe.

Main Results:

Twenty-two out of 96 specimens (22.9%) showed HER-2/neu protein overexpression by IHC.
Only three specimens (3.1%) exhibited HER-2/neu gene amplification by FISH.
No significant correlation was observed between IHC and FISH results.

Conclusions:

The discordance between IHC and FISH suggests that HER-2/neu protein overexpression in GCTs may not have prognostic or therapeutic significance.
The low rate of HER-2/neu gene amplification in GCTs indicates that clinical trials with trastuzumab are not currently recommended.
Further research may be needed to clarify the role of HER-2/neu in GCTs.