Related Experiment Video
Updated: Jan 31, 2026
Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images
Published on: May 19, 2023
[Reproducibility of measurements with the retinal vessel analyzer under optimal conditions]
Mona Pache1, Edgar Nagel, Josef Flammer
1Universitäts-Augenklinik Basel, Germany.
Background:
[corrected] To evaluate the reproducibility of measurements with the Retinal Vessel Analyzer (RVA) in healthy subjects and to describe which measuring conditions should be guaranteed to obtain optimal results.
Methods:
The diameter of retinal arteries and veins of 20 healthy subjects (M : F = 11 : 9; mean age: 33 +/- 12 years) were measured with the RVA at baseline, after 2 hours, and after 2 weeks.
Results:
No statistically significant differences in the diameter of retinal arteries and veins between the single measurements were present. Short-term variability of arterial diameter was 1 %, and the intraclass correlation coefficient kappa was 0.96. Long-term variability was 1.8 %, kappa was 0.98. In retinal veins, a short-term variability of 1 % was calculated, with a kappa of 0.97. Long-term variability was 1.5 %, with a kappa of 0.98.
Conclusion:
Due to the high reproducibility of its measurements, the RVA appears to be a useful device for both analysis and follow-up of retinal vessel diameters.
Related Concept Videos
01:26Optimization Problems
00:48Optimal Foraging
01:15Structure of Blood Vessels
01:20Anatomy of Blood Vessels
Arteries
Arteries circulate oxygenated blood from the heart, except the pulmonary artery, which transports deoxygenated blood to the lungs. Large arteries, such as the aorta,...
01:07Development of Blood Vessels
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
01:14Overview of Blood Vessels
Arteries and Arterioles: Arteries are muscular and elastic vessels that primarily carry oxygenated blood from the heart to body tissues, except for the pulmonary artery, which carries deoxygenated blood. They have thick walls to withstand high pressure and contain a layer of muscle tissue, allowing them to expand or contract as...