Related Experiment Video
Updated: Aug 9, 2026
Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development
Published on: July 13, 2014
Ethanol-induced Cas tyrosine phosphorylation and Fyn kinase activation in rat brain
1Department of Legal Medicine, Osaka Medical College, Daigaku, Takatsuki, Japan.
Background:
Tyrosine phosphorylation signaling has been reported to be involved in regulatory mechanisms of ethanol-induced modulation of the central nervous system.
Methods:
We investigated the effect of ethanol administration on tyrosine phosphorylation signaling in rat brain and also examined the possible involvement of Fyn kinase in the process.
Results:
Immunoprecipitation experiments showed that Crk-associated substrate (Cas) was tyrosine-phosphorylated in response to ethanol administration. Fyn kinase was shown to be activated by ethanol administration and to phosphorylate Cas on tyrosine residue in vitro. Furthermore, Fyn kinase was co-localized with Cas in rat cerebellum and cerebral cortex.
Conclusions:
Cas was tyrosine-phosphorylated in rat brain by ethanol administration, and Fyn kinase was most likely involved in the process.
