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Brain catalase inhibition blocks ethanol-related decrease of blood luteinizing hormone levels in mice.

Carles Sanchis-Segura1, Carlos M G Aragon

  • 1Area de Psicobiologia, Universitat Jaume I, Campus de Borriol, Apartat. 8029 AP, Castelló 12071, Spain.

Alcoholism, Clinical and Experimental Research
|August 29, 2002
PubMed
Summary

Ethanol lowers blood luteinizing hormone (LH) by releasing beta-endorphins. Catalase inhibition blocked this effect, suggesting brain catalase activity mediates ethanol

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Area of Science:

  • Neuroendocrinology
  • Pharmacology

Background:

  • Ethanol consumption is known to decrease luteinizing hormone (LH) levels in rodents.
  • This effect is linked to ethanol-induced beta-endorphin release and subsequent mu-receptor-mediated inhibition of GnRH neurons.
  • Ethanol-stimulated beta-endorphin release in hypothalamic cultures involves the enzyme catalase.

Purpose of the Study:

  • To investigate whether catalase inhibition affects ethanol's impact on blood LH levels.
  • To explore the role of brain catalase activity in mediating ethanol's neuroendocrine effects.

Main Methods:

  • Swiss albino mice were pretreated with varying doses of the catalase inhibitor 3-amino-1,2,4-triazole (AT).
  • Mice subsequently received saline, ethanol, or morphine.
  • Blood LH levels were measured 2 hours after ethanol administration via immunoenzymatic assay.

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Main Results:

  • The catalase inhibitor AT dose-dependently prevented the ethanol-induced reduction in blood LH levels.
  • AT did not affect LH levels in saline or morphine-treated groups.
  • This blockade of ethanol's effect correlated with reduced brain catalase activity.

Conclusions:

  • Catalase inhibition antagonizes the effect of ethanol on blood LH levels.
  • Brain catalase activity plays a significant role in mediating ethanol's impact on LH.
  • Findings suggest a functional link between brain catalase and beta-endorphins in ethanol's psychopharmacological effects.