Alternative processing of endogenous or exogenous antigens extends the immunogenic, H-2 class I-restricted peptide

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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
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Reinhold Schirmbeck1, Jörg Reimann

  • 1Institute of Medical Microbiology and Immunology, University of Ulm, Helmholtzstr 8/1, Albert Einstein Allee 11, D-89081, Ulm, Germany.

Molecular Immunology
|August 30, 2002
PubMed

We investigated the murine, MHC class I-restricted cytotoxic T lymphocyte (CTL) response to a viral antigen delivered by different vaccination strategies to either the endogenous, or an alternative exogenous processing pathway. The immunization techniques used primed distinct (though overlapping) repertoires of CTL epitopes. In vitro studies revealed evidence for the generation of immunogenic, L(d)- and K(b)-binding peptides from endocytosed, exogenous antigen by alternative (endolysosomal) processing. Endogenous antigens expressed by DNA vaccines as a stress protein-associated fusion proteins gains access from the cytosol to endolysosomal processing. Hence, exogenous as well as endogenous protein antigens can gain access to alternative processing pathways and can give rise to an extended repertoire of antigenic epitopes. These studies indicate novel ways for the rational design of vaccine candidates that can prime CTL responses.

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