[Factors determining the antigenic activity of tissue cultures from arbovirus-infected diagnostic specimens]

Formation of arbovirus group A and B antigens is influenced significantly by the number of cells, time of monolayer growth before inoculation, the time of maximum accumulation of hemagglutinins, the infectious dose, the growth and maintenance medium composition, the autointerfering activity of the viruses and their sensitivity to tissue inhibitors. The stability of the hemagglutinating and complement-fixing properties of arbovirus diagnostic preparations does not depend upon the degree of concentration (7-12-fold) and the amount of the stabilizer (2-4%), and storage of group A diagnostic preparations in ampoules under vacuum or in ampoules sealed with air for 2-3 1/2 years (the observation period) does not influence their antigenic activity.