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[Isolation of a third human urokinase (S0 type)]
Wiener Klinische Wochenschrift
|April 18, 1975
Summary
A novel low molecular weight urokinase (S0 type) was isolated from commercial preparations. This S0 urokinase exists independently or forms a complex (S2 type) with S1 urokinase, separable by isoelectric focusing.
Area of Science:
- Biochemistry
- Proteomics
Context:
- Urokinase is a key enzyme in fibrinolysis.
- Commercial urokinase preparations contain multiple forms.
Purpose:
- To characterize a previously unidentified urokinase variant.
- To elucidate the composition of urokinase complexes.
Summary:
- A third urokinase type (S0, MW 22,000) was isolated using gel diffusion and isoelectric focusing.
- S0 urokinase exists freely or as an S2 complex (MW 54,000) with S1 urokinase (MW 32,000).
- Isoelectric focusing demonstrated the dissociation of S2 into S0 and S1 types.
Impact:
- Identifies a new urokinase subtype with potential implications for therapeutic applications.
- Enhances understanding of urokinase heterogeneity and complex formation.
- Provides insights into the purification and characterization of fibrinolytic enzymes.