Related Experiment Videos
Tag-reporter and resin capture--release strategy in oligosaccharide synthesis
1RIKEN, The Institute of Physical and Chemical Research, Wako-shi, Saitama, Japan. yukito@postman.riken.go.jp
Chemistry (Weinheim an Der Bergstrasse, Germany)
|August 31, 2002
Abstract:
Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Real-time reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.