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Related Experiment Video

Updated: Jul 22, 2026

Isolation of Murine Valve Endothelial Cells

Isolation of Murine Valve Endothelial Cells

Published on: August 21, 2014

Temporal Cre-mediated recombination exclusively in endothelial cells using Tie2 regulatory elements.

Anne Forde¹, Rainer Constien, Hermann-Josef Gröne

¹Department of Molecular Immunology, Division of Tumor Immunology, German Cancer Research Center, Heidelberg, Germany.

Genesis (New York, N.Y. : 2000)

|August 31, 2002

Summary

Researchers developed a new inducible Tie2Cre transgenic mouse model for precise gene targeting in endothelial cells. This tool enables efficient investigation of endothelial-specific gene functions in adult mice.

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Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

The Cre/LoxP recombination system is a powerful tool in genetics.
Tissue-specific Cre transgenic mouse lines are essential for studying gene function in specific cell types.
A need exists for inducible systems allowing temporal control of gene manipulation.

Purpose of the Study:

To generate and characterize a novel inducible Cre transgenic mouse line targeting endothelial cells.
To enable temporal and tissue-specific gene manipulation in the endothelium.
To provide a valuable tool for studying endothelial biology in adult mice.

Main Methods:

Generation of a Tie2Cre transgenic mouse line.
Fusion of Cre recombinase with a mutated estrogen receptor (ER(T2)) for inducible activity.
Administration of tamoxifen (estrogen antagonist) orally for 5 weeks to induce recombination.
Utilized an EGFP reporter mouse line to monitor recombination efficiency.

Main Results:

Achieved highly efficient gene recombination exclusively in endothelial cells.
Demonstrated temporal control of recombination using tamoxifen induction.
Confirmed no recombination in uninduced control mice, indicating high specificity.
Validated the system's efficacy across various endothelial cell populations.

Conclusions:

The inducible Tie2Cre mouse line offers precise temporal and spatial control of gene targeting in endothelial cells.
This model is highly beneficial for investigating the roles of endothelial-specific genes in adult mice.
The system allows for efficient induction of transgenes within the endothelium.