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Related Experiment Videos

Ischemia-reperfusion does not cause significant hyaluronan depolymerization in skeletal muscle.

Shayn E Armstrong1, Donald R Bell

  • 1Center for Cardiovascular Sciences, Albany Medical College, New York 12208-3479, USA.

Microvascular Research
|September 3, 2002
PubMed
Summary

Reperfusion of ischemic skeletal muscle did not significantly alter hyaluronan size in tissue. Lymph carried more high-molecular-weight hyaluronan after reperfusion, but depolymerization was minimal.

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Area of Science:

  • Biochemistry
  • Physiology
  • Molecular Biology

Background:

  • Skeletal muscle ischemia and reperfusion can cause tissue damage.
  • Hyaluronan (HA) is a major component of the extracellular matrix.
  • The behavior of hyaluronan during reperfusion is not fully understood.

Purpose of the Study:

  • To investigate if reperfusion of ischemic skeletal muscle leads to hyaluronan depolymerization.
  • To quantify hyaluronan size and content in muscle tissue and lymph during reperfusion.
  • To assess the role of lymph in hyaluronan removal.

Main Methods:

  • Collected prenodal lymph and gastrocnemius muscle from anesthetized rabbits.
  • Measured hyaluronan size using agarose gel electrophoresis and radiometric assay.

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  • Analyzed hyaluronan and albumin content, and tissue hydration.

    • Main Results:

    • Control muscle had high-molecular-weight hyaluronan; lymph had low-molecular-weight hyaluronan.
    • Post-reperfusion lymph showed a 25-fold increase in high-molecular-weight hyaluronan flux.
    • Muscle hyaluronan size/content remained unchanged; lymph albumin flux and tissue edema increased.

    Conclusions:

    • Reperfusion of ischemic skeletal muscle does not cause significant hyaluronan depolymerization in the tissue.
    • Lymphatic removal of hyaluronan is a minor pathway, insufficient to account for substantial depolymerization.
    • Edema and increased albumin transport occur during reperfusion, but hyaluronan integrity is maintained in the tissue.