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Exhaled hydrogen peroxide, nitrite and nitric oxide in healthy children: decrease of hydrogen peroxide by atmospheric
European Journal of Medical Research
|September 3, 2002
Summary
Hydrogen peroxide and nitrite in exhaled breath are potential airway inflammation markers. High environmental nitric oxide (envNO) influences hydrogen peroxide measurements in healthy children, but markers are otherwise unrelated.
Area of Science:
- Respiratory Medicine
- Biomarkers
- Environmental Health
Background:
- Exhaled breath condensate (EBC) contains markers like hydrogen peroxide (H2O2) and nitrite (NO2-) for airway inflammation.
- Understanding factors influencing these markers is crucial for accurate non-invasive assessment.
Purpose of the Study:
- To investigate factors affecting H2O2 and NO2- measurements in EBC.
- To explore correlations between H2O2, NO2-, exhaled nitric oxide (eNO), and environmental nitric oxide (envNO).
Main Methods:
- Assessed H2O2 and NO2- in EBC from 102 healthy children (4-18 years) using fluorometry.
- Evaluated atopy status, lung function, and skin prick tests.
- Measured eNO and envNO via chemiluminescence during EBC sampling.
Main Results:
- Median H2O2: 0.51 µM; Median NO2-: 3.3 µM.
- Significant negative correlation found between H2O2 and envNO (r = -0.50, p < 0.0001).
- eNO was independent of envNO; no other significant correlations observed among markers.
Conclusions:
- H2O2, NO2-, and eNO are not interrelated in healthy children.
- High envNO levels require consideration when interpreting H2O2 measurements in EBC.
- envNO impacts H2O2 measurement, suggesting environmental factors are important for interpreting EBC inflammatory markers.