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[RAPD analysis of germplasm in "baizhi"]
1Instituute of Chinese Material Medica, China Academy of Traditional Chinese Medicine, Beijing 100700.
Objective:
To analyse the germplasm in Baizhi.
Method:
Using 12 random primers, over seventeen individuals' RAPD were detected in four populations from wild Baizhi (Angelica dahurica), Qi Baizhi and Hang Baizhi.
Results:
A total of 40 loci were amplified, 26 of which were polymorphism(65%). The PPS values of Hang Baizhi and Qi Baizhi indicate their low levels of genetic diversity. According to the RAPDinstance analysis, the genetic distance(0.16) between Qi Baizhi and Hang Baizhi is shorter than that of the individuals of the populations of Qi Baizhi and Hang Baizhi, and all of these genetic distances are shorter than those between wild Baizhi and Hang Baizhi(Qi Baizhi).
Conclusion:
Hang Baizhi- and Qi Baizhi belong to the same taxon, which is different from the wild Baizhi.