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Updated: Aug 13, 2026

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Published on: August 23, 2013
[Activation of neutrophil and its relation to neutrophil-endothelial cell adherence]
Abstract:
Neutrophil was activated by chemotactic tripeptide N-formyl-methionyl-leucyl-phenylalanine(fMLP) of different concentration. The activation rate was tested by the method of nitroblue tetrazolium reduction. Using flow chamber system, we quantitatively studied the properties of neutrophil adhesion to human umbilical vein endothelial cell (HUVEC) under different shear stress. The results showed that the activation rate of neutrophil significantly increased with increase of fMLP concentration that the adhesion rate of neutrophil pretreated with fMLP decreased with the gradual increase of shear stress, such a change was also found in the control group; and that the adhesion rate of neutrophil to HUVEC increased with the increase of activation rate of neutrophil, but the adhesion rate had a tendency to decrease when shear stress increased. These indicated that shear stress induced by bloodstream might play an important role in inhibiting neutrophil-endothelial cell adherence in vivo and the enhanced activation of neutrophil could increase neutrophil-endothelial cell adherence.
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