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Updated: Aug 19, 2026
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
[Chemical studies on polysaccharides from mycelium of Hericium erinaceum (Bull. Ex Fr.) Pers]
1Research Laboratory of Chinese Drugs, School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083.
Objective:
To study the chemical composition and structure of polysaccharides from the mycelium of Hericium erinaceum.
Method:
DEAE-cellulose and DEAE-Sephadex column chromatography were employed for isolation and purification. Sephadex column chromatography and agarose gel electrophoresis were used to measure the purity. Gel filtration was employed to measure MW. Acid hydrolysis, enzymolysis, GC and spectral analysis etc. were employed for determination of the composition and construction of the unique homogeneous polysaccharides.
Result:
The five homogeneous polysaccharides were obtained from the mycelium. The main compounds(HEPA1, HEPA4 and HEPB2) were identified.
Conclusion:
HEPA1 (MW 62,000) is a heteropolysaccharide consisting mainly of glucose. HEPA4 (MW 26,000) is a heteropolysaccharide peptide. HEPB2 (MW 12,000) is a heteropolysaccharide consisting mainly of glucose. The main linkage of all the afore-said homogeneous monosaccharides is alpha-linkage.
