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Updated: Sep 29, 2026

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
Published on: March 14, 2016
Turkey and chicken interleukin-18 (IL18) share high sequence identity, but have different polyadenylation sites in
1Institute for Animal Health, Compton, Berkshire RG20 7NN, UK. pete.kaiser@bbsrc.ac.uk
Abstract:
The cDNA of turkey interleukin-18 (IL18) was cloned, initially using oligonucleotide primers based on the sequence of the chicken IL18 gene. The coding regions of the turkey and chicken IL18 genes are highly conserved (96.1% nucleotide identical and 97.4% amino acid identical). The 3' UTR of the turkey IL18 mRNA contains two 'instability' motifs and a canonical polyadenylation site, as compared to the chicken IL18 mRNA which contains only one instability motif and lacks a canonical polyadenylation site. Phylogenetic analysis shows that turkey and chicken IL18 have diverged to a less extent than IL18 from closely related mammalian species. We predict that turkey and chicken IL18 will cross-react in functional assays.
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