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General O-glycosylation of 2-furfuryl alcohol using beta-glucuronidase
Brett D Martin1, Eric R Welsh, Jonathan C Mastrangelo
1Center for BioMolecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA. bdm@cbmse.nrl.navy.mil
Abstract:
beta-Glucuronidase from bovine liver is able to catalyze transfer of several carbohydrates to furfuryl alcohol, an acid-sensitive diene, with transfer yields as high as 84%. Carbohydrates that were transferred in yields of 30% or higher include gluco-, galacto-, xylo-, and fucopyranose. Small variations in the configuration of the substrate hydroxyls lead to large variations in the catalytic behavior of the enzyme in terms of both the initial reaction velocities and the final ratios of transfer-to-hydrolysis. The high transfer yields and surprising nonspecificity towards carbohydrate suggest that the enzyme may be a versatile tool for the general O-glycosylation of dienic alcohols.
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