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Related Experiment Videos

Reversible changes in the nuclear lamina induced by hyperthermia.

Elizabeth A Falloon1, Joseph R Dynlacht

  • 1Department of Radiation Oncology, Indiana University School of Medicine, Indiana Cancer Pavilion, RT 041, 535 Barnhill Drive, Indianapolis, Indiana 46202, USA.

Journal of Cellular Biochemistry
|September 5, 2002
PubMed
Summary

Hyperthermia distorts the nuclear lamina (NL) structure in cells, but rapid NL recovery, especially in thermotolerant cells, may be key to heat-induced cell survival.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Thermotolerance Studies

Background:

  • The nuclear matrix (NM) is a potential target for heat-induced cell death.
  • Heat shock can alter lamin B content, affecting NM structure and protein composition.

Purpose of the Study:

  • Investigate hyperthermia-induced changes in nuclear lamina (NL) structure.
  • Determine the implications of NL structural changes on cell survival in non-tolerant and thermotolerant cells.

Main Methods:

  • Indirect immunofluorescence techniques.
  • Confocal microscopy.
  • Cell heating at 42°C and 45.5°C.

Main Results:

  • Hyperthermia caused dose-dependent invaginations and distortions of the peripheral NL.

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  • Internal lamin B foci remained unaltered by heat.
  • NL structure recovered over time, with faster recovery observed in thermotolerant cells.

    • Conclusions:

    • Initial damage to the peripheral NL does not directly correlate with heat-induced cell killing.
    • A faster rate of NL recovery may confer a survival advantage against hyperthermia.