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Actin-based phagosome motility.

Fangliang Zhang1, Frederick S Southwick, Daniel L Purich

  • 1Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Health Science Center, Gainesville, FL 32610-0245, USA.

Cell Motility and the Cytoskeleton
|September 5, 2002
PubMed
Summary
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Phagosomes containing latex beads exhibit actin-based motility within macrophages, forming actin rocket tails. This movement is comparable to pinosomes, offering new insights into phagocytosis and phagosome maturation.

Area of Science:

  • Cell Biology
  • Immunology
  • Cytoskeletal Dynamics

Background:

  • Actin's role in particle internalization during phagocytosis is established.
  • The intracellular motility of phagosomes, similar to endocytic vesicles or pathogens, remains poorly understood.

Purpose of the Study:

  • To investigate whether phagosomes exhibit actin-based motility within the macrophage cytoplasm.
  • To characterize the nature and dynamics of this motility.

Main Methods:

  • Video microscopy was used to track latex bead-containing phagosomes in bone marrow macrophages.
  • Immunofluorescence microscopy identified actin-rich rocket tails associated with motile phagosomes.

Main Results:

  • Phagosomes containing various latex beads (IgG-opsonized, BSA-coated, uncoated) demonstrated actin-based motility.

Related Experiment Videos

  • Motility was characterized by brief (1-2 min) actin-rich rocket tails.
  • Average speeds ranged from 0.11 to 0.14 microm/s, comparable to pinosome motility.

    • Conclusions:

    • Phagosomes undergo actin-based motility, a novel observation.
    • This motility is transient and associated with actin rocket tails.
    • Findings provide a foundation for studying phagocytosis and phagosome maturation dynamics.