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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
[Determination of buddlejasaponin IV in Clinòpodium Linn. by HPLC]
1Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094.
Objective:
To determine the contents of buddlejasaponin IV in Clinopodium plants.
Method:
A high-performance liquid chromatographic method was used to determine buddlejasaponin IV in Clinopodium plants and Tabellae Clinopodii. The determination was carried out on a C18 reversed-phase column by methanol-water(70:30) elution solvent with UV detection at 210 nm.
Result:
The contents of buddlejasaponin IV in different samples were different. The average recovery was 101.3% and RSD 1.73%.
Conclusion:
The method for determining buddlejasaponin IV was established for the first time.
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