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[Determination of stilbene in radix Polygoni multiflori by HPLC and its stability study]
Abstract
Objective:
To develop a HPLC method for determining stilbene in Radix Polygoni Multiflori and studying its stability.
Method:
HPLC conditions: An Econosphere C18 column, a mixture of methanol-water(30:70) as mobile phase, and UV detection at 310 nm. In addition, the stability of stilbene was studied at high temperature and in acidic solution.
Result:
The linear range of calibration curve was 2.24 x 10(-2)-1.12 x 10(-1) micrograms(n = 6) with a correlation coefficient of 0.99998, the average recovery with RSD being 99.96% +/- 1.54%.
Conclusion:
The method is sensitive, accurate and speedy. Stilbene in aqueous solution is stable, but high temperature(80 degrees C) tends to affect the stability. In acidic solution no stability can be found.