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[Determination of astragaloside IV in radix Astragali by HPLC with evaporative light scattering detector]
Objective:
To develop a new method for the determination of astragaloside IV in Radix Astragali.
Method:
A HPLC-ELSD method was set up, using Hypersil C18 column (150 mm x 4.6 mm), acetonitrile-water(1:2) as mobile phase with a flow rate of 1.0 ml.min-1. The parameters of drift tube and gas flow rate of the detector were set at 105 degrees C and 2.96 L.min-1 respectively.
Result:
The calibration curve was linear in the range of 2.02-10.12 micrograms. The average recovery was 100.5%.
Conclusion:
The active constituent astragaloside IV in Radix Astragali can be separated effectively. ELSD detection is appropriate for the determination of constituents with end absorption at low ultra-violet wavelengths.
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