[Cloning of the recombinant human hepatocyte growth factor gene]

The full length of HGF cDNA gene(2,184 bp) was amplified successfully from human placental tissue using RT-PCR technique, and then cloned into pGEM-T vector, which was identified and confirmed by restriction endonuclease mapping using Nde I, Bg1 II, Hind III, BamH I and Xho I, as well as DNA sequencing. The above clone of HGF cDNA gene was successfully subcloned into the retrovirus vector(pLNL-XHC), which may be used for further studies of gene expression and gene therapy.