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[Experimental study on Jurkat cell apoptosis induced by Boswellia carterii Birdw extractive]
1Department of Hematology, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Objective:
To study the influence of Boswellia carterii Birdw(BCB) extractive of different concentrations on human Jurkat cell apoptosis at different time points.
Methods:
Agarose gel electrophoresis, transmission electron microscope(TEM) and flow cytometry(FCM) were used for observing DNA ladders, morphology of Jurkat cells and cell cycle, respectively.
Results:
BCB induced apoptosis of Jurkat cells and typical DNA ladders; TEM demonstrated the presence of apoptosis Jurkat cells, condensation of cytoplasm, numerous vaculoes in cytoplasm, compaction of the nuclear chromatin and formation of apoptosis dody. The sub-G1 peak was detected by cell cycle analysis. It revealed that G1 phage cells increased and S phage cells decreased.
Conclusion:
The data indicate that BCB extractive induces time- and concentration-dependent apoptosis in Jurkat cell line.