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A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
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A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity

Published on: August 9, 2017

[Study on the optimum experimental conditions for the steady growth of human K-562 cell line]

Y H Huang1, Q Y Xie, D Z Jiang

  • 1Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078.

Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University
|September 6, 2002
PubMed

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media