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Related Experiment Videos

Glucocorticoids modulate transformation by v-Src in a cell-specific manner.

Claudia Provenzano1, Stefano Alemà, Germana Falcone

  • 1Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Via Ramarini 32, Monterotondo Scalo, 00016, Rome, Italy.

Experimental Cell Research
|September 6, 2002
PubMed
Summary

Glucocorticoid hormones like dexamethasone control cell transformation differently in Balb 3T3 and NIH-3T3 cells. This study reveals a new epigenetic mechanism for hormone-driven cell-specific transformation control.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Oncology

Background:

  • The v-src oncogene induces cell transformation, but its regulation is complex.
  • Glucocorticoid hormones are known to influence various cellular processes.

Purpose of the Study:

  • To investigate the role of the glucocorticoid hormone dexamethasone in regulating v-src-induced cell transformation.
  • To elucidate the cell-specific mechanisms underlying dexamethasone's effects on fibroblast transformation.

Main Methods:

  • Infection of Balb 3T3 and NIH-3T3 murine fibroblasts with v-src retroviruses.
  • Treatment with varying doses of dexamethasone.
  • Assays for transformed foci and anchorage-independent colony formation.
  • Analysis of glucocorticoid receptor expression and promoter transactivation.

Related Experiment Videos

  • Cocultivation assays to assess cell-cell interactions and transformation rescue.

    • Main Results:

    • Dexamethasone significantly increased transformation in Balb 3T3 cells but decreased it in NIH-3T3 cells.
    • The effects were dose-dependent and mediated by specific glucocorticoid receptors, with comparable receptor expression and promoter activity in both cell lines.
    • Dexamethasone did not alter v-Src protein expression or kinase activity.
    • The hormone rescued the transformation potential of phenotypically normal v-src-expressing Balb 3T3 cells in cocultivation assays.

    Conclusions:

    • Glucocorticoid hormones play a cell-specific role in controlling oncogene-induced cell transformation.
    • Epigenetic mechanisms are implicated in this hormone-mediated regulation of transformation.
    • These findings suggest novel therapeutic strategies targeting hormone signaling in cancer.