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Oxylipin profiling in pathogen-infected potato leaves
Cornelia Göbel1, Ivo Feussner, Mats Hamberg
1Department of Stress and Developmental Biology, Institute of Plant Biochemistry, Weinberg 3, D-06120, Halle/Saale, Germany.
Biochimica Et Biophysica Acta
|September 6, 2002
Summary
Potato plants activate a defense response involving 9-lipoxygenase (LOX) pathway oxylipins when attacked by pathogens. These compounds accumulate more rapidly during non-disease-causing bacterial interactions than during late blight disease.
Area of Science:
- Plant pathology
- Biochemistry
- Molecular biology
Background:
- Plants possess multicomponent defense responses against pathogen attacks.
- Oxylipins synthesized via the lipoxygenase (LOX) pathway are crucial for plant resistance.
- Potato cells utilize 9-LOX-dependent metabolism of polyunsaturated fatty acids (PUFAs) in response to pathogen elicitors.
Purpose of the Study:
- To investigate the role of 9-LOX-derived oxylipins in potato defense against pathogens.
- To compare oxylipin accumulation in response to different pathogen types (bacterial vs. oomycete).
- To analyze the involvement of jasmonic acid (JA) in potato-pathogen interactions.
Main Methods:
- Oxylipin profiling in potato plants.
- Infection of potato plants with Pseudomonas syringae pv. maculicola and Phytophthora infestans.
- Analysis of linolenic acid (LnA) derivatives, divinyl ethers (CnA, CA), and JA accumulation.
Main Results:
- Potato plants showed increased levels of 9-LOX-derived oxylipins, including trihydroxy-LnA, CnA, CA, and 9-hydroxy-LnA upon pathogen infection.
- Accumulation of these 9-LOX products was faster and more pronounced with P. syringae pv. maculicola (non-disease) compared to P. infestans (late blight).
- Jasmonic acid (JA), a 13-LOX product, accumulated after P. syringae pv. maculicola infiltration but not P. infestans infection.
Conclusions:
- The 9-LOX pathway plays a significant role in potato's early defense response to pathogens.
- Differential accumulation of oxylipins correlates with the outcome of the plant-pathogen interaction (disease vs. resistance).
- Jasmonic acid's role appears distinct from the 9-LOX pathway in these specific potato-pathogen interactions.