Related Experiment Video

Updated: Sep 29, 2026

Model of Ischemia and Reperfusion Injury in Rabbits
06:11

Model of Ischemia and Reperfusion Injury in Rabbits

Published on: November 3, 2023

Early evolution from ischemia to myocardial necrosis

M C Fishbein1

  • 1UCLA School of Medicine, Los Angeles, CA 90095, USA. mfishbein@mednet.ucla.edu

Cardiovascular Toxicology
|September 6, 2002
PubMed

Insights

Pathologic techniques precisely assess myocardial ischemic injury. Electron microscopy detects early injury, while histology quantifies irreversible damage and infarct age, aiding new diagnostic tests and therapies.

Area of Science:

  • Cardiovascular Pathology
  • Ischemic Heart Disease Research

Background:

  • Myocardial ischemic injury diagnosis relies on pathological assessment.
  • Accurate determination of injury presence, age, and extent is crucial for patient outcomes.

Purpose of the Study:

  • To evaluate the utility of pathologic techniques in assessing myocardial ischemic injury.
  • To compare electron microscopy and histology for detecting and quantifying ischemic damage.

Main Methods:

  • Review of electron microscopic studies for early ischemic changes.
  • Analysis of histologic studies for irreversible injury and infarct size measurement.
  • Correlation of morphologic findings with injury age.

Main Results:

  • Electron microscopy detects ischemic injury within minutes, preceding necrosis.
  • Histology identifies irreversible injury hours old and efficiently quantifies infarct size.
  • Histologic methods allow for reliable determination of infarct age.

Conclusions:

  • Pathologic techniques, including electron microscopy and histology, offer valuable insights into myocardial ischemic injury.
  • Morphologic studies are essential for understanding injury progression and aiding the development of diagnostics and therapeutics for ischemic heart disease.

More Related Videos

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery08:38

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Published on: August 16, 2019

Related Concept Videos

Myocarditis I: Introduction01:21

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...
Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...
Cellular Injury IV: Necrosis01:16

Cellular Injury IV: Necrosis

Necrosis is a form of irreversible cell death caused by severe injury such as ischemia, toxins, or trauma. Unlike programmed cell death, it is an uncontrolled, pathological process that typically provokes inflammation in surrounding tissues.Pathophysiologic ChangesNecrosis begins when cells sustain critical damage, leading to swelling of organelles, particularly mitochondria, and rapid ATP depletion. As energy levels decline, membrane ion pumps fail, leading to calcium influx and eventually,...
Acute Coronary Syndrome I: Introduction01:30

Acute Coronary Syndrome I: Introduction

Acute Coronary Syndrome (ACS) encompasses a spectrum of heart conditions caused by sudden obstruction of coronary arteries, typically resulting from the rupture of an atherosclerotic plaque and subsequent thrombus (blood clot) formation. This obstruction can lead to partial or complete blockage of blood flow, causing varying degrees of myocardial ischemia or infarction.ACS includes the following clinical entities:Unstable Angina (UA)Non-ST-Elevation Myocardial Infarction (NSTEMI)ST-Elevation...
Necrosis01:16

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.