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Comparative study on ELISA, IFA and direct methods in diagnosis of giardiasis
Samia M Rashid1, Ibraheim M Nagaty, Amina I Abdel Maboud
1Department of Parasitology, Benha Faculty of Medicine, Benha Branch of Zagazig University, Egypt.
Abstract:
A total of 200 cases aged 1-13 years complaining of symptoms suggestive of giardiasis in addition to thirty normal healthy children as a control group was examined clinically and laboratory using stool examination and detection of Giardia coproantigen in faeces by Dot ELISA and direct IFA. Stool examination detected 39 cases (19.5%) while Dot ELISA and direct IFA detected 49 cases (24.5%) with 100% sensitivity and 93.8% specificity. The common clinical findings were diarrhea, abdominal cramps, anorexia, underweight, pallor and manifestations of vitamin deficiency. The results were discussed.