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Identification of Trichophyton rubrum by PCR fingerprinting
Hongmei Zhu1, Hai Wen, Wanqing Liao
1Cyptoccucus Laboratory, Department of Dermatology, Changzheng Hospital Second Military Medical University, Shanghai 200003, China. hmzhu__cn@yahoo.com.cn
Objective:
To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum,and establish an effective molecular method to identify T. rubrum.
Methods:
Three primers, including (GACA)(4), (GTG)(5) and M13 core sequence (5'-GAGGGTG-GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.
Results:
Different PCR-fingerprinting was seen between T. rubrum and T. mentagrophytes using three different primers. 2 stains of T. rubrum were identified among 6 supposed T. mentagrophytes.
Conclusions:
T. rubrum can be distinguished using PCR, and (GACA)(4) is the most suitable primer.