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High Expression in Insect Cells of A Functional Chimeric Antibody with Specificity for HBsAg
Yong-Jun Yu1, Yu-Lei Jiang, Guan-Zhen Yang
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
Abstract:
We have constructed a double-recombinant virus BacHL4.2 containing both the chimeric heavy- and light-chain cDNAs from monoclonal antibody of the HBsAg gene. Both murine-human chimeric antibody heavy- and light-chain were expressed in Sf9 cells infected with a double-recombinant virus BacHL4.2 or co-infected with separate heavy- and light-chain recombinant viruses. In both cases, expressed products were correctly assembled into normal H(2)L(2) immunoglobulin monomers. ELISA and functional immunoblot assay showed that the recombinant chimeric antibody exhibited a specificity for HBsAg similar to that of the parental murine monoclonal antibody OH3.