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Published on: September 10, 2015
A Study of the Myocardial Nuclear Calcium Transport of Rabbit in vitro
Pei-Yong Wang1, Yong-Zhong Pang, Jing-Yi Su
1Research Laboratory of Pathophysiology, Basic Research Institute of Cardiovascular Disease, Beijing Medical University, Beijing 1OOO83, China.
Abstract:
The characteristics of nuclear calcium regulation were investigated in isolated rabbit myocardial nuclei. It was found that calcium concentration in myocardial nuclei was 2.6 fold more than that in myocardial homogenate (P<0.O1), and the nuclear calcium content was only l/6 of the total cellular calcium. Ca-ATPase of myocardial nuclei was [Ca(2+] and [ATP] dependent. [Ca2+] dependent K(a) and V(max) at 2.O mmol/L [ATP] were 226 nmol/L and 3 460 nmol/(h.mg) protein respectively. [ATP] dependent K(m) value and V(max) at 400 nmol/L [Ca2+] were 376.5 &mgr;mol/L and 2 445 nmol/(h.mg) protein respectively. A positive correlation between nuclear 45)Ca2+ transport and Ca-ATPase activity was observed (r=O.945, P<0.01). The above result suggests that myocardial nuclei are able to transport calcium actively. The pathophysiological role of myocardial nuclear calcium transport should be further determined.
