Related Experiment Video
Updated: Sep 29, 2026
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
Published on: April 5, 2018
Preparation of Unilamellar Immunoliposomes by Passing Reversed Micelles through Oil-water Interface
Zhong-Dang Xiao1, Jian-Hua Gu, Min-Hua Xu
1National Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, China.
A novel method for the preparation of unilamellar immunoliposomes is introduced. In this method, the aqueous phase is first encapsulated into reverse micelles passing through the oil-water interface, where the monolayer of lecithin embedded with antibody has been formed to self-assemble into immunoliposomes. The main advantages of this method are that the procedure of preparation is simple with high encapsulation yield and it is favorable for large scale production. As shown by negative staining electronic micrograph, the immunoliposomes are unilamellar and 100-500 nm in size. The UV spectra of immunoliposomes solution and lysis assay show that sheep anti-human IgG has been coated on liposomes.
A novel method for the preparation of unilamellar immunoliposomes is introduced. In this method, the aqueous phase is first encapsulated into reverse micelles passing through the oil-water interface, where the monolayer of lecithin embedded with antibody has been formed to self-assemble into immunoliposomes. The main advantages of this method are that the procedure of preparation is simple with high encapsulation yield and it is favorable for large scale production. As shown by negative staining electronic micrograph, the immunoliposomes are unilamellar and 100-500 nm in size. The UV spectra of immunoliposomes solution and lysis assay show that sheep anti-human IgG has been coated on liposomes.
