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Studies on the beta(l-4)Galactosyltransferase of Cell Surface in Induced HL60 Cells
Guo-Qiang Wu1, Song-Min Jiang, Jian-Xin Gu
1Gene Research Center, Ministry of Public Health, Key Laboratory of Glycoconjuagte Research, Department of Biochemistry, Shanghai Medical University, Shanghai 200032, China.
Abstract:
A fluorescene assay method for beta(1-4) galactosyltransferase (beta1-4GT) of cell surface has been developed using a pyridylaminated sugar as an acceptor substrate. A fluorescent sugar chain, whose reducing end of the Gnbeta1-2Malpha1-6(Gnbeta1-2Malpha1-3) Mbeta1-4Gnbeta1-4(Fucalpha1-6) Gn has been aminated with 2-aminopyridine. beta1-4GT activity of cell surface varied in different stages of the cell cycle with the highest activity at interphase. The enzyme activity of cell surface took a change when HL60 cell line was induced to differentiate by PMA or RA. The cell surface enzyme activity was 1.30 times as much as tile control group at 24 h in the case of PMA induction, and a maximum increase of 70% over the control on the third day with RA induction.
