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Eukaryotic Expression in COS7 Cells and Biological Activity of Human Brain-derived Neurotrophic Factor (BDNF)
Qian Chen1, Jia-Zheng Wang, Ming Fan
1Institute of Basic Medical sciences, Academey of Military Medical Sciences, Beijing 100850, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|January 1, 1997
Abstract:
In order to study the expression of human brain-derived neurotrophic factor in eukaryotic cells, hBDNF cDNA was cut off from MI3mpl8-hBDNF plasmid and inserted into the eukaryotic expression vector pCMV4. Using lipofectin methods, the recombinant expression plasmid pCMV4-hBDNF was transfected into COS7 cells for transient expression. RNA slot hybridization analysis and immunocytoreaction analysis demonstrated that the BDNF gene can be transcripted and translated in COS7 cells. The human rBDNF secreted by transfected COS7 cells can promote the development and survival of dopaminergic neurons.