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Related Experiment Videos

Biofilm development in down flow anaerobic fluidised bed reactors under transient conditions.

F Tessele1, G Englert, L O Monteggia

  • 1Instituto de Pesquisas Hidráulicas, Universidade Federal do Rio Grande do Sul, Porto. Alegre, Brasil. ftessele@ppgiph.ufrgs.br

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|September 10, 2002
PubMed
Summary

This study investigated biofilm formation on polypropylene particles in an anaerobic fluidized bed reactor. Complete biofilm structure was achieved by day 44, with microbial communities identified.

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Area of Science:

  • Environmental microbiology
  • Biotechnology
  • Anaerobic digestion

Background:

  • Biofilm development is crucial for microbial community establishment in anaerobic reactors.
  • Polypropylene particles serve as a carrier material in fluidized bed reactors.
  • Understanding biofilm formation kinetics is essential for optimizing reactor performance.

Purpose of the Study:

  • To investigate biofilm development on polypropylene particles in a laboratory-scale downflow anaerobic fluidized bed reactor.
  • To characterize the biofilm structure and microbial community.
  • To correlate biofilm characteristics with reactor operational parameters.

Main Methods:

  • Laboratory-scale downflow anaerobic fluidized bed reactor operation.
  • Scanning Electron Microscopy (SEM) for biofilm monitoring and microbial identification.

Related Experiment Videos

  • Atomic Force Microscopy (AFM) for surface roughness analysis.

    • Main Results:

    • Initial cell adhesion occurred within 6 hours.
    • Complete biofilm structure was observed after 44 days.
    • Methanotrix bacilli and Methanosarcina sp. were identified within the biofilm.
    • Biofilm roughness was 9.1 nm, while carrier surface roughness was 75 nm.
    • Good correlation was found between SEM characterization and conventional anaerobic reactor parameters.

    Conclusions:

    • Polypropylene particles effectively support biofilm development in anaerobic fluidized bed reactors.
    • The study identified key microbial populations involved in biofilm formation.
    • SEM and AFM provide valuable insights into biofilm structure and its relationship with reactor performance.