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Related Experiment Videos

Fighting foam with phages?

J A Thomas1, J A Soddell, D I Kurtböke

  • 1Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|September 10, 2002
PubMed
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Seventeen novel phages targeting mycolata were isolated from activated sludge. These phages, belonging to the Siphoviridae family, show diverse host ranges and potential applications in microbial control.

Area of Science:

  • Microbiology
  • Virology
  • Environmental Science

Background:

  • Mycolata, including genera like Gordonia and Rhodococcus, are prevalent in activated sludge.
  • Foaming in activated sludge can be a significant operational problem.
  • Bacteriophages (phages) are viruses that infect bacteria and can be used for microbial control.

Purpose of the Study:

  • To isolate and characterize novel phages infective for mycolata from activated sludge.
  • To assess the host range and diversity of isolated phages.
  • To discuss the potential applications of these phages in managing activated sludge processes.

Main Methods:

  • Isolation of phages from activated sludge using prospective mycolata hosts.
  • Morphological characterization of isolated phages.

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  • Host-range studies using a panel of mycolata strains.

    • Main Results:

    • Seventeen phages infective for mycolata were isolated.
    • All isolated phages belonged to the Siphoviridae family, with variations in head and tail morphology.
    • Host-range studies revealed unique and often polyvalent host ranges for each phage, with many infecting multiple genera.

    Conclusions:

    • Novel mycolata-infective phages were successfully isolated from activated sludge.
    • The isolated phages exhibit significant diversity in their host specificities.
    • These phages hold promise for applications in controlling mycolata populations and mitigating issues like foaming in wastewater treatment.