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Developmental patterning of the circumvallate papilla.
Suwanna Jitpukdeebodintra1, Yang Chai, Malcolm L Snead
1Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles 90033, USA.
The International Journal of Developmental Biology
|September 10, 2002
Summary
Circumvallate papilla development in mouse embryos involves cell migration and innervation, not initial cell division. E-cadherin and laminin may guide nerve ingrowth, influencing gland formation.
Area of Science:
- Developmental Biology
- Molecular Biology
- Histology
Background:
- Organogenesis relies on epithelial-mesenchymal interactions.
- Key molecular players include growth factors, transcription factors, ECM, and cell surface receptors.
Purpose of the Study:
- Investigate molecular mechanisms of circumvallate papilla and von Ebners' gland morphogenesis.
- Examine expression of cell adhesion molecules, ECM, innervation, and cell division during development.
Main Methods:
- Analysis of mouse embryos from embryonic day 11.5 (E11.5) to E15.
- BrdU labeling for cell division assessment.
- Immunohistochemical staining for E-cadherin and laminin.
- Observation of nerve fiber penetration and distribution.
Main Results:
- Cell division is detected later (E14-E15) in the developing papillary area and gland invagination.
- Laminin shows broad expression where nerve fibers penetrate.
- E-cadherin is intensely expressed in specific epithelial layers and where nerve fibers innervate.
- Nerve fibers penetrate the epithelium at E13, guiding gland formation.
Conclusions:
- Circumvallate papilla formation is initially independent of cell division, suggesting a role for cell migration.
- E-cadherin and laminin may function in nerve guidance.
- Innervation significantly impacts the final morphogenesis of the circumvallate papilla and von Ebners' gland.