Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial repression of PCNA by p53 during kidney development.

Zubaida Saifudeen1, Jessica Marks, Hong Du

  • 1Department of Pediatrics, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA.

American Journal of Physiology. Renal Physiology
|September 10, 2002
PubMed
Summary

The transcription factor p53 represses proliferating cell nuclear antigen (PCNA) gene expression during kidney development. This repression is crucial for spatial gene expression and normal kidney structure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding the Role of Base Species on Reversed Cu Catalyst in Ring Opening of Furan Compounds to 1, 2-Pentanediol.

ChemSusChem·2023
Same author

Comparison and improvement of RNA extraction methods in Sargassum (Phaeophyta).

Journal of phycology·2023
Same author

PIWIL2 restrains the progression of thyroid cancer via interaction with miR-146a-3p.

BMC endocrine disorders·2023
Same author

Resolving the dynamics of chrysolaminarin regulation in a marine diatom: A physiological and transcriptomic study.

International journal of biological macromolecules·2023
Same author

Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer.

Cancer gene therapy·2023
Same author

The long transcript of lncRNA TMPO-AS1 promotes bone metastases of prostate cancer by regulating the CSNK2A1/DDX3X complex in Wnt/β-catenin signaling.

Cell death discovery·2023

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Transcriptional repression guides spatial gene expression and cell fate.
  • Proliferating cell nuclear antigen (PCNA) is downregulated during renal epithelial cell differentiation.
  • The transcription factor p53 promotes renal epithelial cell differentiation.

Purpose of the Study:

  • To investigate the impact of p53 inactivation on PCNA expression in mice.
  • To evaluate the effect of p53 on PCNA transcription during kidney development.

Main Methods:

  • Immunohistochemistry to assess PCNA and p53 expression patterns in developing kidneys.
  • Electrophoretic mobility shift assays to study p53 binding to PCNA promoters.
  • Transient transfection assays in p53-deficient cells to analyze p53's effect on PCNA transcription.

Related Experiment Videos

Main Results:

  • PCNA expression pattern is disrupted in p53-deficient mice, with redistribution into the differentiation zone.
  • p53 accumulation correlates with the transition from nephrogenesis to terminal differentiation.
  • p53 represses PCNA transcription, potentially through a mechanism independent of direct DNA binding.

Conclusions:

  • p53-mediated transcriptional repression is proposed to regulate the spatial restriction of PCNA gene expression in normal renal development.
  • Dysregulation of this process in p53-deficient kidneys leads to distorted tubular architecture.
  • p53 plays a critical role in coordinating renal epithelial cell differentiation and growth arrest through PCNA regulation.