Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in urinary excretion of aquaporin-2 associated with impaired urinary concentrating ability in diabetic

Tomoatsu Nakamura1, Takako Saito, Ikuyo Kusaka

  • 1Department of Medicine, Division of Endocrinology and Metabolism, Jichi Medical School, Tochigi, Japan.

Nephron
|September 10, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The long-term dialysis due to diabetic nephropathy may be associated with in-hospital death in patients with acute myocardial infarction.

Heart and vessels·2026
Same author

Impact of right bundle branch block in anterior ST-segment elevation myocardial infarction in the contemporary primary PCI era.

Heart and vessels·2026
Same author

Inhibition of MAFB and PI3K/AKT Signaling for Hereditary FSGS with Multicentric Carpotarsal Osteolysis.

Journal of the American Society of Nephrology : JASN·2026
Same author

Mechanisms of Symptomatic Lower Extremity Artery Disease in Femoropopliteal Arteries Assessed by Optical Frequency Domain Imaging.

The American journal of cardiology·2026
Same author

Efficacy for LDL-C-lowering and safety of pemafibrate extended-release formulation in patients with statin-intolerant hypercholesterolemia: A phase 3 multicenter, randomized, double-blind, placebo-controlled, parallel-group trial.

Journal of clinical lipidology·2026
Same author

Comparison of Clinical Outcomes in Patients With Acute Myocardial Infarction Between Those With and Without Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD).

Circulation reports·2026

Diabetic nephropathy impairs urinary concentration by reducing aquaporin-2 (AQP-2) excretion. This suggests arginine vasopressin (AVP) signaling is affected in kidney collecting duct cells, contributing to renal failure.

Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • Aquaporin-2 (AQP-2) is crucial for renal water reabsorption in collecting ducts, regulated by arginine vasopressin (AVP).
  • Diabetic nephropathy is a common complication of diabetes, often leading to chronic kidney disease and impaired urinary concentrating ability.

Purpose of the Study:

  • To investigate the relationship between renal dysfunction progression and urinary AQP-2 excretion in patients with type 2 diabetes.
  • To determine if impaired AVP cellular signaling contributes to urinary concentrating defects in diabetic nephropathy.

Main Methods:

  • Study included 8 controls and 14 type 2 diabetes patients with varying renal function (serum creatinine cut-off: 1.5 mg/dL).
  • Measurements included urinary osmolality, plasma osmolality, AVP levels, and urinary AQP-2 excretion after water deprivation and water loading.

Related Experiment Videos

Main Results:

  • Diabetic patients with renal failure showed significantly decreased urinary osmolality and AQP-2 excretion after water deprivation compared to controls.
  • Following a water load, urinary AQP-2 excretion remained significantly lower in diabetic patients with renal failure, despite similar plasma osmolality and AVP levels.
  • No significant differences in plasma osmolality or AVP levels were observed between groups after water loading.

Conclusions:

  • Decreased urinary AQP-2 excretion in diabetic nephropathy is linked to impaired AVP cellular signaling in collecting duct cells.
  • This impaired signaling may contribute to the urinary concentrating defect observed in diabetic kidney disease and renal failure.