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Deoxyribonucleic acid contamination in archival human temporal bones: a potentially significant problem
Michael J McKenna1, Arthur G Kristiansen, Anke S Tropitzsch
1Department of Otology and Laryngology, Harvard Medical School, Boston, Massachusetts, USA. mjm@epl.meei.harvard.edu
Summary
Extraneous DNA contaminates archival human temporal bones, leading to inaccurate genetic analysis. Future studies should include blood samples from donors to ensure reliable deoxyribonucleic acid (DNA) data.
Area of Science:
- Genetics
- Molecular Biology
- Otolaryngology
Background:
- Archival human temporal bones are valuable for studying pathologic changes using molecular techniques.
- These specimens offer a unique repository of historical data not available from living patients.
Observation:
- Analysis of an archival temporal bone for a specific mutation yielded normal deoxyribonucleic acid (DNA) sequences in most attempts.
- The known mutation was readily detected in a blood sample from the same individual.
Findings:
- Three out of four DNA extractions from the archival temporal bone showed normal sequences, inconsistent with the known mutation.
- The discrepancy suggests contamination with exogenous DNA during specimen acquisition or processing.
Implications:
- Contamination of archival temporal bones with extraneous DNA is a significant issue impacting genetic analysis.
- Collecting donor blood samples alongside temporal bones is recommended for reliable DNA sourcing and accurate research outcomes.