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Expression of Chicken Smooth Muscle Myosin Light Chain Kinase in NIH 3T3 Cells
Yuan Wang1, Shu-Yu Gui, D.M. Watterson
1Department of Biochemistry and Molecular Biology Laboratory, Anhui Medical University, Hefei 230032, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|January 1, 1997
Abstract:
The myosin light chain kinase (MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.