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Updated: Sep 29, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Published on: July 1, 2018
Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants
Robert Hasterok1, Tim Langdon, Steve Taylor
1Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland. hasterok@us.edu.pl
Abstract:
This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.
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