Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyhydroxy alkaloids: chromatographic analysis.

Russell J Molyneux1, Dale R Gardner, Lynn F James

  • 1Western Regional Research Center, Agricultural Research Service, USDA, Albany, CA 94710, USA. molyneux@pw.usda.gov

Journal of Chromatography. A
|September 11, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is hemp (<i>Cannabis sativa</i>) safe to feed pregnant sheep?

Translational animal science·2025
Same author

An Evaluation of the Toxic Norditerpenoid Alkaloid Content Across Delphinium Species, and Their Acute Toxicities in a Murine Model.

Chemistry & biodiversity·2025
Same author

DNA data (genome skims and metabarcodes) paired with chemical data demonstrate utility for retrospective analysis of forage linked to fatal poisoning of cattle.

Toxicon : official journal of the International Society on Toxinology·2025
Same author

Comparison of the acute toxicity of zygacine versus zygadenine.

Toxicon : official journal of the International Society on Toxinology·2024
Same author

Hair loss and endocrine dermatosis on horses raised in a Mimosa pudica var. unijuga-invaded area.

Toxicon : official journal of the International Society on Toxinology·2024
Same author

Analysis of the Mycotoxin Levels and Expression Pattern of SWN Genes at Different Time Points in the Fungus <i>Slafractonia leguminicola</i>.

Microorganisms·2024

This review details chromatographic techniques for isolating and analyzing polyhydroxy alkaloids, natural products that inhibit glycosidases. These methods address challenges like high water solubility and lack of UV detection for these valuable compounds.

Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Analytical Chemistry

Background:

  • Polyhydroxy alkaloids are a diverse group of natural products, including pyrrolidine, piperidine, pyrrolizidine, indolizidine, and tropane classes.
  • These compounds are potent glycosidase inhibitors, indicating significant therapeutic potential.
  • Their structural characteristics (multiple hydroxyl groups) lead to high water solubility and poor solubility in organic solvents, complicating isolation and analysis.

Purpose of the Study:

  • To present effective chromatographic techniques for the isolation, purification, detection, and analysis of polyhydroxy alkaloids.
  • To address the analytical challenges posed by the physicochemical properties of these compounds, such as poor UV detectability.

Main Methods:

  • Review of various chromatographic techniques, including High-Performance Liquid Chromatography (HPLC) and Thin-Layer Chromatography (TLC).

Related Experiment Videos

  • Discussion of detection methods suitable for non-chromophoric, polar compounds.
  • Exploration of sample preparation and purification strategies tailored for polyhydroxy alkaloids.

    • Main Results:

    • Successful application of specific chromatographic methods for separating complex mixtures of polyhydroxy alkaloids.
    • Demonstration of techniques enabling the detection and quantification of these challenging natural products.
    • Identification of optimal solvent systems and stationary phases for effective purification.

    Conclusions:

    • Chromatographic techniques are crucial for overcoming the analytical hurdles associated with polyhydroxy alkaloids.
    • The reviewed methods provide a foundation for further research into the biological activities and applications of these natural products.
    • Advancements in chromatography facilitate the discovery and development of novel glycosidase inhibitors from natural sources.