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Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
Published on: November 10, 2018
First isolation of largemouth bass virus
John M Grizzle1, Ilhan Altinok, William A Fraser
1Department of Fisheries and Allied Aquacultures, Auburn University, Alabama 36849, USA. jgrizzle@acesag.auburn.edu
Diseases of Aquatic Organisms
|September 11, 2002
Summary
Largemouth bass virus (LMBV) was first identified in Florida in 1991, not South Carolina in 1995. Genetic analysis confirmed the Florida and South Carolina LMBV strains are identical.
Area of Science:
- Ichthyology
- Virology
- Molecular Biology
Background:
- Largemouth bass virus (LMBV) is an iridovirus responsible for fish kills, first reported in South Carolina in 1995.
- LMBV has since been detected in numerous locations across the southeastern USA, affecting both diseased and healthy fish populations.
Purpose of the Study:
- To determine the initial geographical origin of Largemouth Bass Virus (LMBV).
- To compare LMBV isolates from South Carolina and Florida using molecular techniques.
Main Methods:
- Comparative analysis of viral DNA using restriction fragment length polymorphisms (RFLP).
- DNA sequencing of a portion of the major capsid protein gene for both isolates.
Main Results:
- Restriction fragment length polymorphisms and DNA sequence data were identical for the LMBV isolates from South Carolina and Florida.
- The findings indicate that LMBV was present in Florida in 1991, predating its first reported occurrence in South Carolina in 1995.
Conclusions:
- Largemouth bass virus (LMBV) was first discovered in Florida, not South Carolina.
- The continued use of the name "largemouth bass virus" is recommended over geographically specific alternatives.
