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Updated: Sep 29, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis: purification, characterization, and
Shin-ichi Miyoshi1, Yuka Sonoda, Hiroko Wakiyama
1Faculty of Pharmaceutical Sciences and Okayama University, Tsushima-Naka, Okayama, 700-8530, Japan
Abstract:
An exocellular metalloprotease produced by Vibrio fluvialis, an enteropathogenic vibrio, was purified and characterized. The metalloprotease (V. fluvialis protease [VFP]) was found to have very similar characteristics to V. vulnificus protease, including a molecular mass of 45kDa, sensitivity to chelating agents or competitive inhibitors for thermolysin-like metalloproteases, and the substrate specificity. The structural gene for VFP was also cloned, and its nucleotide sequence was determined. The deduced amino acid sequence confirmed that VFP was a member of the thermolysin family. VFP, like V. vulnificus protease, showed the haemagglutinating, permeability-enhancing and haemorrhagic activities in addition to the proteolytic activity toward oligopeptide, casein or elastin.

