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Activation of latent pseudorabies virus infection in mice treated with acetylcholine
Seiichi Tanaka1, Kazuaki Mannen
1Animal Laboratory Center, Oita Medical University, Hasama-machi, Oita 879-5593, Japan.
Experimental Animals
|September 12, 2002
Abstract:
Pseudorabies virus (PrV) YS-81 strain latently infected in 6-week-old BALB/c mice was detectable by nasal swabbing, and serum antibody was shown to increase in titer after intraperitoneal injection for 3 days with acetylcholine or dexamethasone.
Insights
Pseudorabies virus (PrV) infection in mice can be detected via nasal swabs. Acetylcholine or dexamethasone treatments increased serum antibodies in latently infected mice.
Area of Science:
- Veterinary Virology
- Immunology
- Animal Models
Background:
- Pseudorabies virus (PrV) establishes latent infections in animal hosts.
- Understanding PrV latency and host immune responses is crucial for disease control.
Purpose of the Study:
- To investigate the detectability of latent Pseudorabies virus (PrV) in mice.
- To evaluate the effect of acetylcholine and dexamethasone on serum antibody titers in latently infected mice.
Main Methods:
- Latent infection model using Pseudorabies virus (PrV) YS-81 strain in 6-week-old BALB/c mice.
- Detection of PrV by nasal swabbing.
- Measurement of serum antibody titers following 3-day intraperitoneal injection of acetylcholine or dexamethasone.
Main Results:
- Latent Pseudorabies virus (PrV) was detectable in infected mice using nasal swabs.
- Serum antibody titers significantly increased after intraperitoneal administration of acetylcholine.
- Serum antibody titers also showed an increase following dexamethasone treatment.
Conclusions:
- Nasal swabbing is a viable method for detecting latent Pseudorabies virus (PrV) infection.
- Acetylcholine and dexamethasone can modulate the immune response, leading to increased serum antibody levels in latently infected mice.