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Updated: Sep 29, 2026

Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
Published on: May 25, 2022
Molecular characterisation of Victorian Newcastle disease virus isolates from 1976 to 1999
I Peroulis-Kourtis1, K O'Riley, D Grix
1Department of Natural Resources and Environment, Victorian Institute of Animal Science, Attwood. irene.peroulis@nre.vic.gov.au
Objective:
To characterise Newcastle disease virus isolates obtained in Victoria from 1976 to 1999 and identify the diversity of FO cleavage signal.
Design:
RT-PCR using viral RNA extracted from positive NDV allantoic fluid was performed to amplify a segment of the NDV F and HN genes. Molecular characterisation of the nucleotide and amino acid sequences within the FO cleavage site was undertaken.
Results:
All isolates contained 'avirulent FO cleavage signal sequence of varied amino acid composition.
Conclusions:
Molecular characterisation of past and present NDV FO cleavage signal sequences will provide valuable epidemiological information and assist in understanding the genetic origins and relationships of outbreaks.
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