Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genotype analysis using human hair shaft.

Hsueh-Wei Chang1, Ching-Yu Yen, Shyun-Yui Liu

  • 1The Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
|September 12, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrence-free survival is prolonged in high-grade serous ovarian cancer with high PD-L1 expression.

BMC cancer·2026
Same author

Unprecedented rearranged triterpenoids with seven-membered rings from <i>Camellia formosensis</i> and structurally diverse bioactive triterpenoids.

RSC advances·2026
Same author

Plumbagin sensitizes leukemia cells to cisplatin by promoting oxidative stress, apoptosis, and DNA damage.

International journal of medical sciences·2026
Same author

Incidental [<sup>68</sup>Ga]Ga-DOTATATE uptake in bone metastases of breast carcinoma in a patient with a secondary carcinoid tumor.

EJNMMI research·2026
Same author

A saliva-based surrogate associates with clinical outcome of oral potentially malignant disorders.

British journal of cancer·2026
Same author

Santamarine Synergizes With Cisplatin via ROS/JNK Axis to Selectively Induce Apoptosis and DNA Damage in Oral Cancer Cells In Vitro.

Drug development research·2026

Genotyping diseases is now easier using DNA from hair shafts. This noninvasive method provides sufficient genetic material for reliable analysis, advancing molecular epidemiology.

Area of Science:

  • Genetics
  • Molecular Epidemiology
  • Biochemistry

Background:

  • Genotyping is crucial for understanding genetic disease predisposition and molecular epidemiology.
  • Current methods for DNA extraction can be invasive or complex.

Purpose of the Study:

  • To develop a convenient and noninvasive method for genotyping using terminal hair shafts.
  • To assess the feasibility of high-throughput genotyping from hair shaft DNA.

Main Methods:

  • DNA was purified from terminal cephalic hair shafts.
  • Genotyping was performed using molecular beacons to detect single nucleotide polymorphisms.
  • Analysis focused on APO-E and G-protein beta3 (GNB3) subunit C825T loci.

Main Results:

Related Experiment Videos

  • Sufficient DNA was obtained from hair shafts for genotyping (average 112 pg/mg).
  • High-throughput genotyping of APO-E and GNB3 loci was successfully performed.
  • The method reliably distinguished between different alleles.

Conclusions:

  • Terminal hair shafts offer a convenient and reliable source of genomic DNA for genotyping.
  • This noninvasive approach has significant potential for clinical applications and molecular epidemiology studies.