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Updated: Sep 29, 2026

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Published on: April 19, 2017
Enumeration, phenotyping, and identification of activation events in conjugates between T cells and
Kristie M Grebe1, Terry A Potter
1Integrated Department of Immunology, National Jewish Medical and Research Center and the University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, CO 80206, USA.
Abstract:
Microscopic analysis of T cell-antigen-presenting cell (T cell:APC) interactions at the single cell level has been a powerful, but tedious and subjective, technique. In this paper, we describe a rapid and quantitative method to identify T cell:APC conjugates using succinimidyl ester dyes, which irreversibly label free amine groups on the cell surface. The labeled cell conjugates and subsequent activation events are detected by flow cytometry.
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